Monday, May 14, 2007

Cultivation of Algae Strains for Biodiesel

Like plants, algae require primarily three components to grow: sunlight, carbon-di-oxide & water. Like plants again, they use the sunlight for the process of photosynthesis. Photosynthesis is an important biochemical process in which plants, algae, and some bacteria convert the energy of sunlight to chemical energy. This chemical energy is used to drive chemical reactions such as the formation of sugars or the fixation of nitrogen into amino acids, the building blocks for protein synthesis. (see Photosynthesis – from Wikipedia). Algae capture light energy through photosynthesis and convert inorganic substances into simple sugars using the captured energy.

Finding algea strains to grow isn't too difficult. Cultivating specific strains of algae for biodiesel could be however a bit more difficult, as they can require high maintenance and could get easily contaminated by undesirable species.

Since algae need for their growth sunlight, carbon-di-oxide and water, they can be cultivated in open ponds & lakes. Due to the fact that these systems are "open", they are much more vulnerable to being contaminated by other algal species and bacteria. The real challenge with open air bioreactors (like a pond) is that the species of algae that have the highest oil content are not necessarily the quickest to reproduce. This creates a problem where other species take over the pond. Undesirable algal species taking over specific strains is one of the more significant problems in algaculture, with the possible exception of spirulina which in of itself is extremely aggressive and also grows at a pH that is extremely high, thereby eliminating the possibliity of contamination to some extent. For this reason, the number of species that have been successfully cultivated for a given purpose in an open system is relatively small. In addition, in open systems there is relatively less control over water temperature, carbon-di-oxide concentration & lighting conditions. These imply that the growing season is largely dependent on location and, aside from tropical areas, is limited to the warmer months. While the above are the disadvantages with “open systems”, some of the benefits of this type of system are that it is one of the cheaper ones to produce - at the most basic you only need to dig a trench or pond.

A variation on the basic "open-pond" system is to close it off, to cover a pond or pool with a greenhouse. While this usually results in a smaller system, it does take care of many of the problems associated with an open system. It allows more species to be able to be grown, it allows the species that are being grown to stay dominant, and it extends the growing season, only slightly if unheated, and if heated it can produce year round. It is also possible to increase the amount of carbon-di-oxide in these quasi-closed systems, thus again increasing the rate of growth of algae.

The ponds in which the algae are cultivated are usually what are called the “raceway ponds”. In these ponds, tha algae, water & nutrients circulate around a racetrack. With paddlewheels providing the flow, algae are kept suspended in the water, and are circulated back to the surface on a regular frequency. The ponds are usually kept shallow because the algae need to be exposed to sunlight, and sunlight can only penetrate the pond water to a limited depth. The ponds are operated in a continuous manner, with CO2 and nutrients being constantly fed to the ponds, while algae-containing water is removed at the other end.

Alternatively, algae could be grown in closed structures called photobioreactors, where the environment is better controlled than in open ponds. While the costs of setting up and operating a photobioreactor would be higher than for those for open ponds, the efficiency and higher oil yields from these photobioreactors could be significantly higher as well, thus offsetting the initial cost disadvantage in the medium and long run.

Photobioreactors

A photobioreactor is an equipment that is used to harvest algae. A photobioreactor is basically a bioreactor that incorporates some type of light source. The term photobioreactor is more commonly used to define a closed system, as opposed to an open pond. A pond covered with a greenhouse could also be considered a photobioreactor. Because these systems are closed everything that the algae need to grow, (carbon dioxide, water and light) need to be introduced into the system.

Photobioreactors can be set up to be continually harvested (the majority of the larger cultivation systems), or by harvesting a batch at a time (like polyethlyene bag cultivation). A batch photobioreactor is set up with nutrients and algal seed, and allowed to grow until the batch is harvested. A continuous photobioreactor is harvested either continually, as daily, or more frequently.

Some types of photobioreactors include:
· glass or plastic tubes
· tanks
· plastic sleeves or bags

Growing algae at home

Take a container and add a small amount of algae culture. If your plans for growing algae are towards producing biodiesel feedstock, you will need to find specific algae strains. Adding an aquarium bubble stone increases growth and circulates the algae. The only requirements for this type of system are CO2, (ambient CO2 is sufficient, though you're growth rate will be slower), nutrients, such as fertilizer or manure, and a light source. The optimal temperature range will depend on the strain you are using.

Some questions found at a message board re growing algae at home: Is it possible to grow the required algae on a small scale? How much know-how do you need? How high are the initial costs? Would growing algae be very complicated? Could they even grow it indoors?..Do you know of any websites that give instructions for do-it-yourself biodiesel (or even ethanol?)...

See also:
Can Oil-producing Algae be Grown at Home? – Biodiesel Now Forum, In-home Photosynthetic Bio-reactor

Lighting

Some sources that can be used to provide the light energy required to sustain photosynthesis include

· Fluorescent bulbs
· LEDs, or
· Natural sunlight

Some more thoughts on algae growth and cultivation

· It could also be worth thinking about how (or if) marine algae could be grown – perhaps through iron fertilization - in otherwise unproductive (high-nitrogen-low-chlorophyll) regions of the open oceans.

Research on Algae Cultivation

· The NREL (national Renewable Energy Laboratory, part of the Department of Energy) conducted research into algae production. NREL favoured unlined “raceway” ponds which were stirred using a paddle wheel, and had carbon dioxide bubbled through it. The water used for these ponds is wastewater (treated sewerage) freshwater, brackish water, or salt water, depending on the strain of algae grown. The algae should be a native to the region.
· Other countries, notably Japan, are interested in closed systems; however these systems (at least from NREL perspective) are very expensive.

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